But, hGO tubes significantly (P less than 0.01) increased cell viability % as compared to hGO rods and hGO sheets. Cell viability percentage was significantly (P less than 0.01) decreased at dose 100 µg/ml and it was significantly (P less than 0.01) increased at 25 µg/ml dose as compared to 50, 10 and 0 µg/ml doses. Result: All three hGO nano structures were damaged cell morphology at 100 and 50 µg /ml doses, however, morphologically more good cells were observed in hGO tubes treated group than hGO rods and hGO sheets at 25 and 10 µg/ml doses as compared to control. Methods: Caprine Wharton’s jelly derived mesenchymal stem cells (WJ-MSCs) were isolated, characterized and dose dependent (100, 50, 25, 10 and 0µg /ml) in vitro cytotoxicity of three different hGO nano structures (phosphorus doped graphene oxide titanium oxide tubes, rods and sheets) were analysed in caprine WJ-MSCs by studying cell cytotoxicity assays. Hybrid GO (hGO) nano structures biocompatibility is depends on its size, dose and exposure time as well as in vitro cell models and hence, need thorough cytotoxicity studies in different species in vitro cell models. Graphene oxide (GO) is a carbon based nanomaterial and it has large surface area as well as good biocompatibility and heteroatoms doped GO exploit its properties. In summary, this review highlights the numerous opportunities and challenges associated with the use of MG-NCs as intelligent nanocomposites (NCs) in tissue engineering and regenerative medicine.īackground: Nanotechnology is used in stem cell culture as well as in vivo delivery and tracking of stem cells. In addition, the scientific and technical aspects of MG-NC used in medicine are discussed considering the requirements for the field. Advanced applications of magnetic nanocomposites (MG-NCs), specifically magnetic nanostructures, are further systematically reviewed. Here, we review, classify, and discuss various synthesis strategies for novel MNSs used in medicine. There have been many reports concerning the role of these nano-building blocks in the regeneration of both soft and hard tissues, but the subject has not been extensively reviewed. Based on the most recent findings, magnetic nanostructures (MNSs), in particular, have emerged as promising materials for detecting, directing, and supporting tissue regeneration. Nanotechnology has further aided tissue regeneration science and has provided outstanding opportunities to help disease diagnosis as well as treat damaged tissues.

Tissue engineering and regenerative medicine have solved numerous problems related to the repair and regeneration of damaged organs and tissues arising from aging, illnesses, and injuries.

Caprine engineering